A rapid enzymatic assay for methotrexate in serum.
نویسنده
چکیده
A kinetic enzymological assay for methotrexate in serum is described; the results are available after 1.5 hours. The lower limit of sensivity is 50 nmol/1; the day-to-day variation coefficients compare favourably with other procedures (about 7% in the 200-600 nmol/1 range). The method uses purified dihydrofolic acid reductase from bovine liver. The problem of its instability in the assay is circumvented by several measures, e.g. the use of a reaction rate analyser.
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ورودعنوان ژورنال:
- Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
دوره 17 4 شماره
صفحات -
تاریخ انتشار 1979